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. 2020 Jul 3;11(7):500. doi: 10.1038/s41419-020-2711-x

Fig. 6. Overexpression of PIK3CG accounted for miR-1976 function in TNBC.

Fig. 6

a The mRNA expression of PIK3CG was measured by qRT-PCR. b The protein expression levels of PIK3CG, AKT, and p-AKT were measured by western blot. The properties of EMT in SUM-1315 and ZR-75-1 transfected with miR-NC, miR-mimics, miR-inhibitor, p-PIK3CG, or PIK3CG inhibitor CAY10505 were determined by wound healing assay (c), transwell assay (d). Scale bars: 100 μm. e p-PIK3CG restored the protein expression of the EMT markers and transcription factors, and CAY10505 counteracted the effect of miR-1976 inhibitor. CSC properties of SUM-1315 and ZR-75-1 transfected with miR-NC, miR-mimics, miR-inhibitor, p-PIK3CG, or PIK3CG inhibitor CAY10505 were determined by flow cytometry analysis (f) and mammosphere formation assay (g). Scale bars: 100 μm. h p-PIK3CG restored the protein expression of CD44, and CAY10505 decreased the expression. *p < 0.05, **p < 0.01, ***p < 0.001. The data expressed as the mean ± SD.