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. 2020 Jul 3;10:11017. doi: 10.1038/s41598-020-67291-0

Figure 1.

Figure 1

Hippocampal neurons in NBCn1 KO mice develop intracellular acidosis. (A) Resting pHi. Resting pHi was determined after steady-state pHi was established in the presence of 5% CO2, 33 mM HCO3− (n = 15 neurons from 8 WT postnatal mice and 23 neurons from 11 KO postnatal mice). (B) Comparison of pHi recovery from a CO2-induced acid load in WT neurons and KO neurons. Recovery of pHi was measured in a CO2/HCO3−-buffered solution containing 10 μM of ethyl isopropyl amiloride to inhibit endogenous Na+/H+ exchangers. (C) Rate of pHi recovery (dpH/dt). Values of dpH/dt (×10−4 pHi/sec) were measured during the first 2 min after CO2-induced acidification (n = 31 WT neurons and 28 KO neurons). **p < 0.01 compared to WT neurons.