Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 3;10:11017. doi: 10.1038/s41598-020-67291-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Loss of NBCn1 increases sensitivity to alcohol-induced sedation and induces the development of tolerance to the sensitivity. (A,B) Loss of righting reflex (LORR) in alcohol-naïve mice. Mice were intraperitoneally injected with 3.0 (n = 4/group) or 3.5 g/kg alcohol (n = 10/group) and subjected to LORR. LORR latency (A) is the time elapsed between alcohol injection and onset of sedation, and LORR duration (B) is the time elapsed between onset of sedation and recovery from LORR. Recovery was considered when mice regained an ability to place all four limbs on a V-shape trough twice within a 30-second period. *p < 0.05 compared to WT mice. (C) Blood alcohol concentration (BAC) after alcohol injection. Blood samples were collected from the submandibular vein at the indicated time-points after an intraperitoneal injection of 2.0 g/kg alcohol (n = 6 WT & 5 KO mice). (D,E) LORR in alcohol-experienced mice. Mice were daily injected with 2.0 g/kg alcohol for 7 days and then 3.0 or 3.5 g/kg of alcohol on day 8 (n = 4–6/group). Mice were then subjected to LORR. Latency (D) and duration (E) are described as above. **p < 0.01 compared to WT mice. (F) Comparison of LORR duration between alcohol-naïve vs. alcohol-experienced mice. Durations in B and E were compared to display the effects of alcohol pretreatment on the sensitivity to alcohol-induced sedation in KO vs. WT mice. **p < 0.01 compared to WT mice.