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. 2020 Jul 3;10:11003. doi: 10.1038/s41598-020-67411-w

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Displays the various cell populations gated for F4/80 + macrophages and CD3⁺ T cells. CD3⁺ T-cell subsets were then fractioned by CD4 and CD8 positivity. (b) Shows the frequencies of immune cells observed in the GL261 and GL261 Red-FLuc implanted brains dissociated into a single cell suspension 10 days post-implantation. (c) Displays a panel of immune cell frequencies observed in GL261 and GL261-Luc2 implanted brains. Statistical significance was determined by Mann–Whitney U test with P-values of less than 0.05 considered statistically significant. All images in this figure were made using GraphPad Prism 8.3.0 (580) https://www.graphpad.com/scientific-software/prism/, Microsoft PowerPoint Office 365 https://www.microsoft.com/enus/p/powerpoint/cfq7ttc0k7c6?activetab=pivot:overviewtab and FlowJo v10.6.2 https://www.flowjo.com/solutions/flowjo/downloads.