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. 2020 Jul 3;10:11003. doi: 10.1038/s41598-020-67411-w

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Representative protein microarray images display differential cytokine profiles and raw pixel density for each group, respectively. (b) Relative fold change was determined and shown for cytokines of interest. The data are presented as means ± SEM, n = 3 for each group with (*P < 0.05, **P < 0.01, ***P < 0.001) by One-way ANOVA. Bar graphs show differential protein expression for selected cytokines of interest. (c) TGF-β2 ELISAs results show upregulated TGF-β2 expression measured in pg/mL for GL261 Red-FLuc and GL261-Luc2 compared to GL261 cells. All images in this figure were made using GraphPad Prism 8.3.0 (580) https://www.graphpad.com/scientific-software/prism/ and Microsoft PowerPoint Office 365 https://www.microsoft.com/enus/p/powerpoint/cfq7ttc0k7c6?activetab=pivot:overviewtab.