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. 2020 Jul 3;11:3303. doi: 10.1038/s41467-020-17150-3

Fig. 4. LIN28B-knockout cells are less aggressive in mouse models.

Fig. 4

a Schematic of two complementary animal tumor metastasis models utilizing human cells placed within immunocompromised mice. b Representative luminescent images and quantification of lungs explanted at sacrifice from mice injected with crCTRL1 (nonsense gRNA transduced) or crLIN28B knockout (transduced with gRNA targeting LIN28B exon 3) PANC1 cells. *p = 0.001, 2-sided t-test, n = 8 per group, median and 25–75% IQR shown by box plots. Scale bar is 1 cm. c Representative time course of images acquired and quantification of whole animal bioluminescent signals after orthotopic implantation of either crCTRL1 or crLIN28B exon 4 knockout PANC1 cells. *p = 0.035, Mann–Whitney U test, n = 7 per group; mean ± SEM for each time point shown.