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. 2020 Jul 3;10:10991. doi: 10.1038/s41598-020-67841-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Killing of silkworms by injection of T. asahii. Saline or T. asahii JCM2466 (2 × 10⁶ cells) was injected into the silkworm hemolymph. Survival of the silkworms at 27 °C (A) and 37 °C (B) was monitored. n = 10/group. (C) Silkworms after injection at 48 h under 37 °C incubation are shown. (D) Saline or T. asahii JCM2466 (2 × 10⁶ cells) was injected into the silkworm hemolymph. Silkworm hemolymph was collected at 0, 6, and 24 h after injection and observed with a microscope. White arrow indicates hemocytes of silkworm. Black arrowheads indicate T. asahii cells.