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. 2020 Jul 3;10:10991. doi: 10.1038/s41598-020-67841-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Death of silkworms according to the number of T. asahii cells administered. (A) Saline or T. asahii JCM2466 (6.7 × 10⁴ – 1.8 × 10⁷ cells) was injected into the silkworm hemolymph. Survival of the silkworms at 37 °C was monitored. n = 6/group. (B) Survival of the silkworms at 48 h was monitored. The LD₅₀, which is the dose of T. asahii required to kill half of the silkworms, was determined from combined data of six independent experiments.