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. 2020 Jul 3;10:10991. doi: 10.1038/s41598-020-67841-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Therapeutic effects of antifungal drugs in silkworms infected with T. asahii. (A) Saline or T. asahii JCM2466 (3 × 10⁶ cells) was injected into the silkworm hemolymph, followed by injection of 50 µl of amphotericin B (AMPH-B, 100 µg/ml), fluconazole (FLCZ, 100 µg/ml), or voriconazole (VCZ, 100 µg/ml) into the hemolymph. Silkworms were reared at 37 °C and survival of the silkworms was monitored. (B) Silkworm hemolymph was collected at 24 h after injection and observed with a microscope.