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. 2020 Jul 3;10:10991. doi: 10.1038/s41598-020-67841-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of transgenic T. asahii expressing eGFP. (A) Schematic illustration of gene transfer of the nourseothricin resistance gene NAT1 and the gene encoding eGFP to the T. asahii JCM2466 strain. (B) JCM2466 strain and JCM2466 eGFP-Tg strains were spread on Sabouraud agar medium containing nourseothricin (50 µg/ml) and incubated at 27 °C for 2 days. (C) Detection of gene encoding eGFP in the chromosome of the T. asahii strain by PCR. Full-length gels are presented in Supplementary Fig. 5. (D) The JCM2466 strain and the T. asahii eGFP-Tg strain were observed with a fluorescence microscope. BF: bright field. Flu: fluorescent, which is field of view under conditions of irradiation with excitation light for fluorescence detection.