Figure 7.

Antifungal drug-induced inhibition of growth of transgenic T. asahii expressing eGFP in silkworm hemolymph. (A) Saline or T. asahii JCM2466 eGFP-Tg strain (3 × 10⁶ cells) was injected into the silkworm hemolymph, followed by injection of 50 µl of amphotericin B (AMPH-B, 100 µg/ml), fluconazole (FLCZ, 100 µg/ml), or voriconazole (VCZ, 100 µg/ml) into the hemolymph. Silkworm hemolymph was collected at 24 h after injection and observed with a fluorescence microscope (A). The fluorescence was calculated (B). Statistically significant differences between groups were evaluated using Tukey–Kramer method. *P < 0.05.