Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used in the study.
| Primers | Nucleic acid sequence |
|---|---|
| pAg1-NAT for infusion cloning | |
| FpAg1-NAT | AGGATCCTTGCGCGCCTAGGC |
| RpAg1-NAT | GAAGAGATGTAGAAACTAGCT |
| CnPactin for infusion cloning | |
| FCnPactin(-pAg1-NAT) | TTTCTACATCTCTTCGCTGCGAGGATGTGAGCTGGA |
| RCnPactin(-eGFP) | CTCGCCCTTGCTCACCATGCCTCGATGGCCTCGGCGTCC |
| eGFPTtrpC for infusion cloning | |
| FeGFPTtrpC(-CnPactin) | TTTCTACATCTCTTCATGGTGAGCAAGGGCGAGGAG |
| ReGFPTtrpC(-pAg1-NAT) | GCGCGCAAGGATCCTAAAGAAGGATTACCTCTAAACAAGTGTACC |
