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. 2020 Jul 3;10:11007. doi: 10.1038/s41598-020-67691-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence characterization of cortical cultures in simple and complex systems at DIV31. Neurons were identified by staining for Tuj-1 (Neuron-specific class III beta-tubulin, a, e). Glial fibrillary acidic protein (GFAP) was used to identify astrocytes (b, f) and myelin basic protein (MBP) was used to identify mature oligodendrocytes and myelin (white arrowheads) (c, g). Merged images with nuclear stain (DAPI, blue) are shown in (d) and (h). Figure has been modified to remove electrode autofluorescence. Scale bar = 50 µm.