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. 2020 Jul 3;10:11007. doi: 10.1038/s41598-020-67691-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Single-cell RNAseq data. (a) t-SNE plot showing eleven different cell clusters. Different cell types (and sub-types) are color-coded. (b) t-SNE plot of all cells for both simple and complex groups at each DIV. Gene markers for specific cell types are shown in (c–h); Tubb3 and Nrgn for neurons (c, d); Gfap and Aqp4 for astrocytes (e, f); Pdgfra and Mbp for oligodendrocytes (g, h).