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. 2020 Jul 3;10:11007. doi: 10.1038/s41598-020-67691-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Baseline characteristics of simple and complex cultures. Two representative electrodes with neuronal activity are shown for simple and complex recordings in (a) and (b) at DIV31, respectively. Comparisons of activity and firing features over days in vitro (DIV) are shown including: (c), % active electrodes, (d), firing rate, (e), % spikes in bursts, (f), burst duration, (g), interspike interval within bursts (ISI) and (h), synchrony. Data are shown as mean ± SEM. Asterisks (*) indicate significance in two-way ANOVA. *p < 0.05, **p < 0.01. Asterisks associated with DIV indicate significance for culture type. Asterisks alone indicate significance for media type.