Fig. 3. AD and DS bind to PHF23_PHD and inhibit the survival of NUP98–PHF23 expressing AML cells.

a Chemical structures of amiodarone and disulfiram. b, c Superimposed ¹H,¹⁵N HSQC spectra of PHF23_PHD collected upon titration with AD or DS. Protein concentration was kept at 100 μM. Concentrations of AD were 0, 0.5, and 2 mM b. Concentrations of DS were 0, 0.1, and 0.2 mM c. Spectra are color coded according to the protein:compound molar ratio. d, e AD treatment affected the survival of NUP98–PHF23 (748T) and control (7298/2) cell lines. For additional trials see ref. ⁹. Source data are provided in the Source Data file. f Concentration dependent effect of DS on the survival of NUP98–PHF23 expressing 961C, NUP98–HOXD13 expressing 189E6, and 32D AML cell lines. For additional trials see ref. ⁹. Source data are provided in the Source Data file. Identification of the AD-binding g and DS-binding h sites of PHF23_PHD. Residues that exhibit significant ligand-induced resonance perturbations in b, c are mapped onto the structure of PHF23_PHD. See also Supplementary Fig. 4. i Samples containing 0.1 mM PHF23_PHD were incubated with indicated amounts of DS for 10 min, 30 min, 1 h, 3 h, and overnight (o/n). All samples were flash-frozen and resolved by SDS-PAGE under nonreducing and reducing (10 mM mercaptoethanol (β-ME)) conditions. Experiment was repeated independently two times with similar results.