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. 2020 Jul 3;11:3339. doi: 10.1038/s41467-020-17098-4

Fig. 6. NUP98–KDM5A is enriched at H3K4me3 regions and is sensitive to DS.

Fig. 6

a ChIP-seq profiles of H3K4me3, NUP98–KDM5A (N5A, two replicates), and H3K27me3 at the Hoxa, Meis1, and Hoxb loci in murine leukemia cells transformed by NUP98–KDM5A. b Heatmap of H3K4me3 and two replicas of NUP98–KDM5A ChIP-seq signals centered on H3K4me3-binding sites in a ±5.0-kb window in murine leukemia cells expressing FLAG-tagged NUP98–KDM5A. ChIP-seq signals were normalized to input. c DS induces cell death of NUP98–KDM5A expressing and NUP98–PHF23 expressing AML cell lines at earlier time points and lower DS concentrations than the NUP98–HOXD13 expressing cell line, confirming our previous results9. n = 1, source data are provided in the Source Data file. Superimposed 1H,15N HSQC spectra of BPTFPHD d and KDM5APHD e collected upon titration with DS. Spectra are color coded according to the protein:compound molar ratio. Dashed box indicates newly appeared cross-peaks.