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. 2020 Jul 3;3:340. doi: 10.1038/s42003-020-1059-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Number of DMR-associated (blue circle) and differentially expressed genes (green circle) between Himchana 1 and JGK 3 at S3 and S5 stages of seed development are given in Venn diagrams. b Number of differentially (hyper/hypo) methylated and differentially (up/down) expressed genes in different sequence contexts and genic regions between Himchana 1 and JGK 3 at S3 and S5 stages of seed development are shown. Intensity of mustard and blue colors indicate number of DMR-associated upregulated and downregulated genes, respectively. c, d Differential methylation (DM) and differential expression (DE) of the genes between JGK 3 and Himchana 1 at S3 (c) and S5 (d) stages of seed development given in b are shown via heatmaps. Scales at the bottom represent percentage of methylation level difference and differential expression in log₂ fold-change. e Heatmap showing hypermethylation in CG context within gene body in JGK 3 (as compared to Himchana 1) associated with higher expression at S3 and/or S5 stages of seed development for sets of genes involved in cell cycle, cell growth, grain filling processes and genes located within known QTLs-associated with seed size/weight in chickpea. Scales at the bottom represent percentage of methylation level difference and differential expression in log₂ fold-change. U upstream, GB gene body, D downstream.