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. 2020 Jul 3;11:3363. doi: 10.1038/s41467-020-17094-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic experimental overview. HUVEC = human umbilical vein endothelial cell. b Growth curve showing replication of Ot in cultured HUVEC cells. Bacteria were grown in 24-well plates and the total bacterial genome copy number per well as measured by qPCR is shown. Bacteria were added at an multiplicity of infection (MOI) of 8:1 (UT176) and 25:1 (Karp). Mean and SD from three independent replicates are shown. c Confocal microscopy images of Ot in HUVEC cells 5 days post infection. Additional images and time points are shown in Supplementary Figs. 1 and 2. Blue = DAPI (DNA), Red = Evans blue (host cells), green = Ot labeled with Alexa488-click-methionine. Scale bar = 5 µm. d RNA mapping statistics showing the fraction of host and Ot RNA for each condition. The first replicate of the experiment is shown. Individual results for each replicate are shown in Supplementary Fig. 3. e Percentage of RNA-seq reads assigned to different classes of RNA in Karp, UT176 and HUVEC. Source data are provided as a Source Data file.