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. 2020 Jul 3;11:3363. doi: 10.1038/s41467-020-17094-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Northern blot analysis of core non-coding RNAs in Ot, showing results of three independent biological replicates. b Structure of the two-piece tmRNA observed in the Ot transcriptome. c RNA-seq read coverage over the tmRNA gene mirrors cleavage observed by Northern blot. d A comparison of genomic synteny of two species within the enterobacteriaceae (Escherichia coli MG1655 and Salmonella enterica serovar Typhimurium SL1344, top), with synteny between the two Orientia strains from this study (bottom). e Pie charts illustrating the relative abundance of RAGE genes in conserved (top) and strain-specific (bottom) operons. f Visualization of the largest conserved operon in Ot, encoding multiple ribosomal genes, showing RNA-seq coverage in both strains. Source data are provided as a Source Data file.