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. 2020 Jul 3;11:3327. doi: 10.1038/s41467-020-17148-x

Fig. 5. In vivo monocyte/macrophage lineage differentiation of GCase-targeted HSPCs.

Fig. 5

a Distribution of B-lymphoid and myeloid lineage cells within the engrafted human cell population in the bone marrow from mice transplanted with CD68S-GCase and CD68S-GCase-P2A-Citrine-targeted HSPCs (n = 29, 31 mice). b Distribution of B-lymphoid and lineage cells within the engrafted human cell population from secondary transplants. Empty: vector without Citrine (n = 5 mice). c Representative FACS plots showing Citrine expression in human CD33+ (Myeloid), CD14+ (Monocyte) and CD19 (B-cells). d Percent Citrine-positive cells in monocyte (black), myeloid (white), and B-cell (gray) populations in mice with human CCR5 allele modification fraction >10%. e Representative epifluorescence microscopy images of human CD68S-GCase-P2A-Citrine-targeted macrophages differentiated from human CD14+ cells sorted from mice bone marrow and peripheral blood (n = 10 mice, additional examples in Supplementary Fig. 8). Images depict morphology (brightfield), nuclei (Hoechst, blue), CD68 protein (red), and Citrine (green). Scale bar is 10 µm. a, b, d Median shown. Source data are provided as a Source Data file.