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. 2020 Jul 3;11:3326. doi: 10.1038/s41467-020-17181-w

Fig. 1. Glutamine supplementation inhibits melanoma tumour growth.

Fig. 1

a Nude mice with subcutaneous injection of M229 cells received control or high glutamine (High Gln) diet 1 week post injection. Tumours were measured twice weekly (Control, n = 6; High Gln, n = 5). b Final tumour volumes at day 59 in the control and High Gln diet groups (Control, n = 6; High Gln, n = 5 biologically independent tumours). c M229 xenograft tumour weights post mortem (Control, n = 6; High Gln, n = 5 biologically independent tumours). d Glutamine concentration from M229 tumours and nude mice serum measured by EIA kit (n = 6 for Control and n = 4 for High Gln biological replicates). e, f Body weight in e and food intake in f of nude mice injected with M229 xenografts (Control, n = 6; High Gln, n = 5). g Serum from M229 tumour-bearing (TB) and non-TB nude mice fed control or glutamine-supplemented (High Gln) diet. Blood was collected by cardiac puncture and analysed for liver and kidney function by EIA kit. Dashed lines represent expected levels in nude mice (Control non-TB n = 2, Control TB n = 4, High Gln non-TB n = 4, High Gln TB n = 4, biological replicates performed in triplicates). h B6.BRafCA, PtenloxP, Tyr::CreERT2 tri-allelic transgenic mice placed on control or glutamine-supplemented (High Gln) diet 1 week post 4-hydroxytamoxifen topical administration until clinical end point. p value calculated by Mantel–Cox test. i B6.BRafCA, PtenloxP, Tyr::CreERT2 tumour areas post mortem (Control, n = 6; High Gln, n = 5 biologically independent tumours). Data represent means and error bars are s.d. p value calculated by t test (unpaired, two tailed) except in b by Mann–Whitney test (unpaired, one tailed). n.s. not significant.