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. 2020 Jul 3;10:10999. doi: 10.1038/s41598-020-67929-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of the workflow of plasma proteomic analysis. The study schema is illustrated with the patient populations and methods used. Plasma protein biomarker discovery (N = 8) was guided by plasma‐based proteomics followed by 2D-DIGE mass spectrometry. Selected protein candidates were validated in an independent cohort (N = 28) by western blotting analysis.