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. 2020 Jul 3;10:10999. doi: 10.1038/s41598-020-67929-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of selected differentially expressed proteins using independent validation cohorts. Immunoblot analysis using of complement component C6 proteins (A), ceruplasmin (B) and haptoglobin (C). Equal protein loading was confirmed with Coomassie staining of plasma samples. Densitometry analysis of each protein normalized to β-actin represents the relative protein expression values. Error bars represent standard error of the mean. *p 0.05, **p 0.01, unpaired t-test. n = three replicates of three independent experiments for each group.