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. 2020 Jul 3;10:10986. doi: 10.1038/s41598-020-67755-3

Figure 6.

Figure 6

Double knockout of Bax and Bak augments early UPR signaling in islets under ER-stress. (a) Quantification of basal Xbp1s and Chop expression in untreated WT and Bax-Bak DKO islets. Quantification of the time-dependent expression of Xbp1s (b) and Chop (c) mRNA in WT and Bax-Bak DKO islets treated with 25 mM glucose + 1.5 mM palmitate (G + PA) or 25 mM glucose + BSA control (G + BSA) for 24 and 48 h. G + PA results are normalized against their respective control conditions for each time-point (represented by the white bar; n = 3–7 for each time-point). Quantification of the time-dependent expression of Xbp1s (d) and Chop (e) in WT and Bax-Bak DKO islets treated with 100 nM Thapsigargin (Tg) compared to DMSO vehicle control (white control bar; n = 4–16 for each time-point). Data represent mean ± SEM. *p < 0.05; ns = not significant. Statistical comparisons were done using 1-way ANOVA with Bonferroni post-hoc tests.