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. 2020 Jul 3;10:10970. doi: 10.1038/s41598-020-67616-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI) assay of (A) control, (B) 2% CHX and (C) 1% QAS specimens; the images shown are consistent with the results of membrane analysis. CLSM images showing (D) 2%CHX and dead bacterial sites around the (E) 1% and (F) 2% QAS molecule attracted due to its surfactant effect. (G) CLSM images and viability of dual specie biofilms treated with different antimicrobial agents, after which, biofilms were stained using the BacLight LIVE/DEAD viability stain.