Fig. 2. IL-10 deletion in T_H17 enhances antibacterial immunity.

T_H17 derived IL-10 impairs the development of an efficient immunity against S. aureus. a A schematic depiction of the experimental plan. b Colony-forming units (CFUs) of S. aureus from different organs. Data are cumulative of two independent experiments. Each dot represents one mouse (n_{wild type} = 7, n_KO = 7). Mean ± SEM.; *P < 0.05 by Mann–Whitney U test. c Flow cytometric analysis of small intestinal CD4⁺ T cells after S. aureus infection. Small intestinal lymphocytes were isolated from the indicated mouse lines and intracellular staining for both IL-17A and IFN-γ was performed to identify T_H17 (IL-17A⁺ IFN-γ⁻), T_H1/T_H17 (IL-17A⁺ IFN-γ⁺) and T_H1 (IL-17A⁻ IFN-γ⁺) cells. A pre-gate on CD4⁺ T cell is applied. One representative experiment out of three is shown. d, e Statistical analysis of frequencies (d) as well as numbers (e) are reported. One representative experiment out of three is shown. Each dot represents one mouse (n_{wild type} = 5, n_KO = 5). Mean ± S.D.; ns, not significant; *P < 0.05 by Mann–Whitney U test. Source data are provided as a Source data file.