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. 2020 Jun 30;13:6295–6302. doi: 10.2147/OTT.S253082

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© 2020 Liu et al.

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SLC7A11-AS1 silencing inhibited proliferation, migration and invasion of lung cancer cells in vitro. (A) The relative SLC7A11-AS1 expression in H1299 and A549 cells of si-SLC7A11-AS1-1 group and si-SLC7A11-AS1-2 group was both markedly decreased than that of si-NC group. (B) Relative to H1299 and A549 cells of si-NC group, those of si-SLC7A11-AS1-1 group and si-SLC7A11-AS1-2 group exhibited significantly lower OD value at 72 h. (C and D) H1299 and A549 cells of si-SLC7A11-AS1-1 group and si-SLC7A11-AS1-2 group showed less number of migrated and invaded cells than si-NC group. (E) The wound healing assay was performed to observe the migration ratio. (F) Western blotting assay was used for detecting E-cadherin and N-cadherin expression. The relative protein levels were calculated. (G) Compared with si-NC group, H1299 and A549 cells of si-SLC7A11-AS1-1 group and si-SLC7A11-AS1-2 group showed much higher relative caspase-3 activity. (H) Apoptosis was analyzed by staining with annexin V/PI through flow cytometry. Apoptotic cells were defined as annexin V and PI double positive. *P < 0.05.