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. 2020 Jun 30;13:2037–2052. doi: 10.2147/IDR.S255292

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© 2020 Dehbashi et al.

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Analytical sensitivity of real-time PCR for primers used to detect class A β-lactamases genes in clinical isolates of P. aeruginosa. ai to aiii: bla_TEM gene; ai: Melting curve in 82.4°C; aii: Amplification curve; and aiii: Standard curve with efficiency=1.429. bi to biii: bla_SHV gene; bi: Melting curve in 88.52°C; bii: Amplification curve; and biii: Standard curve with efficiency=1.742. ci to ciii: bla_KPC gene; ci: Melting curve in 83.55°C; cii: Amplification curve; and ciii: Standard curve with efficiency=1.575. The mean of a: 10⁸; b: 10⁷; c: 10⁶; d: 10⁵; e: 10⁴; f: 10³; g: 10²; h: 10¹ and i: 10° CFU/mL of DNA dilutions. Horizontal lines represent cycle threshold of Real-time PCR. One peak with a shoulder corresponds to genomic DNA amplification; no peak corresponds to no amplification. SYBR Green I Dye and single-tube reaction were used in this test. Also, Real-Time PCR was performed as single-step.