Figure 3.

GRHL2 increased cisplatin-resistance, promoted cell proliferation and retarded cell apoptosis in vitro. (A, C) CCK-8 analysis was used to determine the cell viability and the IC₅₀ values of DDP in SKOV3/DDP-shGRHL2 and SKOV3/DDP-shcontrol cells. *P<0.05 vs SKOV3/DDP-shcontrol. (B, D) CCK-8 analysis was used to evaluate the cell viability and the IC₅₀ values of DDP in SKOV3-GRHL2 and SKOV3-control cells. *P<0.05 vs SKOV3-control. (E) CCK-8 assays were performed to determine the effects of GRHL2 knockdown on the proliferation of SKOV3/DDP cells. *P<0.05 vs SKOV3/DDP-shcontrol. (F) CCK-8 assays were performed to determine the effects of GRHL2 upregulation on the proliferation of SKOV3 cells. *P<0.05 vs SKOV3-control. (G, H) Apoptosis of SKOV3/DDP-shGRHL2 and SKOV3/DDP-shcontrol cells was evaluated by FCM. *P < 0.05 vs SKOV3/DDP-shcontrol. (I, J) Apoptosis of SKOV3-GRHL2 and SKOV3-control cells by FCM. *P<0.05 vs SKOV3-control.

Note: Data were expressed as mean±SD.

Abbreviations: CCK-8, Cell Counting Kit-8; IC₅₀, half-maximal inhibitory concentration.