Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 30;12:5181–5200. doi: 10.2147/CMAR.S250661

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Xu et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

LACTB can mediate EMT and proliferation of CRC cells by regulating autophagy. (A–C) The results showed that LACTB-overexpressing SW480 and HCT116 cells exhibited increased expression levels of P62 and Twist1 after treatment with the autophagy inhibitor MHY1485, but these levels were reduced in LACTB-knockdown LOVO cells treated with the autophagy activator Torin1. In addition, the LC3-II/LC3-I expression ratio was decreased in LACTB-overexpressing SW480 and HCT116 cells following MHY1485 treatment, but this ratio was increased in LACTB-knockdown LOVO cells treated with Torin1. The inhibition of autophagy is associated with an increased level of P62, which prevents the degradation of Twist1 (*P<0.05), as determined by Student’s t-test. (D) Transwell assays revealed that cell migration and invasion were promoted after the attenuation of autophagy in LACTB-overexpressing SW480 and HCT116 cells treated with the autophagy inhibitor MHY1485, whereas LOVO-knockdown cells treated with the autophagy activator Torin1 showed poor cell migration and invasion abilities.