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. 2020 Jun 30;13:6265–6277. doi: 10.2147/OTT.S249994

Figure 3.

Figure 3

Inhibition of cells by cisplatin was enhanced and the level of p- protein kinase B was suppressed by silencing Golgi phosphoprotein 3 in A549 cell, while overexpression of Golgi phosphoprotein 3 had opposite effects.

Notes: (A) The transfection efficiency of Golgi phosphoprotein 3, and (B, C) cell viability. (D) The mRNA expression level and (E) protein level of Golgi phosphoprotein 3 after GOLPH3 silencing was determined. (F) The transfection efficiency of Golgi phosphoprotein 3, and (G) cell viability. (H) The mRNA expression level and (I) protein level of Golgi phosphoprotein 3 after GOLPH3 silencing was determined. (J–K) The protein levels of p-protein kinase B, protein kinase B, p-p65 and p65 were determined by Western blot. **P<0.01 vs. Control, #P<0.05, ##P<0.01 vs. NC, &&P<0.01 vs. NC+Cis, ^P<0.05, ^^P<0.01 vs. Mock, @@P<0.01 vs. Mock+Cis.

Abbreviations: GOLPH3, Golgi phosphoprotein 3; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; OD, optical density; MTT, methyl thiazolyl tetrazolium; NC, negative control; si, silencing; Cis, cisplatin; AKT, serine/threonine protein kinase; pAKT, phosphorylated AKT; P65, nuclear factor kappa B p65; p-P65, phosphorylated P65.