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. 2020 Jun 30;13:6265–6277. doi: 10.2147/OTT.S249994

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© 2020 Zhao et al.

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XAV939 decreased Golgi phosphoprotein 3 overexpression-induced proliferation and promoted cisplatin-induced growth inhibition and apoptosis in H23 cells.

Notes: (A) The viability of cells was tested by MTT. (B) Apoptosis was detected by flow cytometry. (C) In vitro angiogenesis experiment was used to evaluate angiogenesis rates. (D) Images of apoptosis were captured. (E) Images of angiogenesis were taken (magnification x100). The mRNA levels of vascular endothelial growth factor (F), Bcl-2-associated X protein (G) and B cell lymphoma/leukemia-2 (H) were tested by real time quantitative polymerase chain reaction. (I, J) The protein levels of vascular endothelial growth factor, Bcl-2-associated X protein and B cell lymphoma/leukemia-2 were determined by Western blot. ^^P<0.01 vs. Mock, ^aP<0.05, ^aaP<0.01 vs. GOLPH3, ^bP<0.05, ^bbP<0.01 vs. Cis, ^ccP<0.01 vs. XAV939+Cis. Abbreviations: GOLPH3, Golgi phosphoprotein 3; OD, optical Density; MTT, methyl thiazolyl tetrazolium; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Cis, cisplatin; VEGF, vascular endothelial growth factor; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; Annexin-V-FITC, fluoresceine isothiocyanate; PI, propidium iodide.