Fig. 5. SOCS3 impairs IL-2 receptor signaling in Foxp3⁺ Treg cells.

(A) Intracellular pSTAT5 contents in WT and SOCS3^Tg LN Treg cells that were stimulated with IL-2 stimulation (0.3 ng/mL) for 30 min. Histogram is representative (left), and graph shows summary (right) of 2 independent experiments with total 3 WT and 3 SOCS3^Tg mice.

(B) Foxp3⁺ CD25⁺ Treg cells were identified among WT and SOCS3^Tg CD4⁺ LN T cells by intracellular staining (left). Frequency and number of Treg cells (right) were determined from 2 independent experiments with total 5 WT and 5 SOCS3^Tg mice. Data are shown as mean +/− SEM; *P <0.05, **P<0.01, ***P<0.001.