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PCR primer sequences for 5-HT system genes.
| Gene | Primer sequences 5’→3’ | Function |
|---|---|---|
| β-actin | F: TTCCTTCCTGGGTATGGAAT R: GAGGAGCAATGATCTTGATC |
Cytoskelelon protein (housekeeping gene) |
| HTR1A | F: ACGTTACTAGCATCTCCGAC R: CTTGTTGAGCACCTGGTACA |
Gi/Go coupled 5-HT autoreceptor |
| TPH2 | F: AAATATGGCCAGCCCATTCC R: GCATGAGTGGGGTAGAGTTT |
Rate-limiting enzyme in 5-HT synthesis |
| SLC6A4 | F: AGTTCTACTTGCGCCATGTC R: GACGCCTTTCCAGATGCTAA |
5-HT transporter |
