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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Cancer Res. 2020 Mar 25;80(13):2848–2860. doi: 10.1158/0008-5472.CAN-19-3033

Figure 7. RNF168 is required for RAD51 loading in BRCA1 null cells.

Figure 7.

(A) Western blot showing the effects of scrambled (Sc) and two independent siRNAs targeting RNF8, RNF168 and PALB2 in MDA-MB-436 and SUM1315MO2 cell lines.

(B) Cells from (A) were assessed for RAD51 IRIF and quantified as described in Fig. 6A. Inset, representative images.

(C) Cells from (A) were assessed for colony formation. Mean ± S.E.M. are shown normalized to Sc treated cells, **p < 0.01, ***p < 0.001 (unpaired t-test) compared to Sc cells.

(D) Model for the role of RNF168 protein expression levels in regulating residual HR in BRCA1 null cancers.