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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Mol Cancer Res. 2020 Apr 3;18(7):1018–1027. doi: 10.1158/1541-7786.MCR-19-1082

Figure 4.

Figure 4.

Nuclear p65 and p50 are required for the survival of tamoxifen-tolerant cells. A-C, Western blot analysis of nuclear (A) or whole cell lysates (B) of MCF-7 cells treated as in Fig. 1A are shown. TBP or β-actin served as loading controls. Densitometry quantitation is shown (C). N-Nuclear, T-total. D, Clonogenic assay was conducted in MCF-7 parental wild type (WT) or in p65 or p50 CRISPR/Cas9 knock out clones. *P<0.01, **P<0.005.