Table 1.
PDAC Organoid Invasion and Protein Expression Patterns
| Time Lapse Invasion Analysis | Immunofluorescence Analysis | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Number (%) of Organoids with Invasion Pattern | Number (%) of Organoids with Expression Pattern | |||||||||
| Case | SMAD4 Mutation Status | SMAD4 IHC Status | Collective | Mesenchymal | Non-invasive | Organoid Invasion Pattern | CK+ | VIM+ | CK+ / VIM+ | Organoid Protein Expression Pattern |
| PCO1 | wild-type | intact | 0 (0) | 45 (83) | 9 (17) | mesenchymal | ND | ND | ND | ND |
| PCO2 | wild-type | lost | 5 (12) | 34 (83) | 2 (5) | mesenchymal | ND | ND | ND | ND |
| PCO3 | frameshift | lost | 21 (72) | 5 (17) | 3 (10) | collective | 24 (96) | 1 (4) | 0 (0) | epithelial |
| PCO4 | deletion | lost | 35 (76) | 8 (17) | 3 (7) | collective | 8 (100) | 0 (0) | 0 (0) | epithelial |
| PCO5 | wild-type | intact | 0 (0) | 15 (52) | 14 (48) | mesenchymal | 1 (5) | 18 (82) | 3 (14) | mesenchymal |
| PCO6 | wild-type | intact | 0 (0) | 48 (89) | 6 (11) | mesenchymal | 2 (15) | 9 (69) | 2 (15) | mesenchymal |
| PCO7 | deletion | lost | 17 (31) | 9 (16) | 29 (53) | collective | 4 (80) | 1 (20) | 0 (0) | epithelial |
| PCO8 | wild-type | intact | 3 (4) | 58 (73) | 18 (23) | mesenchymal | 6 (17) | 11 (31) | 18 (51) | mesenchymal |
| PCO9 | wild-type | lost | 12 (20) | 36 (61) | 11 (19) | mesenchymal | 17 (85) | 3 (15) | 0 (0) | epithelial |
| PCO10 | deletion | lost | 49 (62) | 17 (22) | 13 (16) | collective | 21 (75) | 1 (4) | 6 (21) | epithelial |
| PCO11 | wild-type | intact | 1 (3) | 29 (81) | 6 (17) | mesenchymal | 2 (13) | 12 (75) | 2 (13) | mesenchymal |
| PCO12 | deletion | lost | 3 (5) | 28 (50) | 25 (45) | mesenchymal | 4 (16) | 18 (72) | 3 (12) | mesenchymal |
| PCO13 | wild-type | lost | 1 (2) | 25 (51) | 23 (47) | mesenchymal | 2 (20) | 7 (70) | 1 (10) | mesenchymal |
| PCO14 | deletion | lost | 9 (20) | 31 (67) | 6 (13) | mesenchymal | 6 (20) | 18 (60) | 6 (20) | mesenchymal |
| PCO15 | wild-type | intact | 10 (12) | 24 (28) | 52 (60) | mesenchymal | 13 (43) | 0 (0) | 17 (57) | mesenchymal |
| PCO16 | wild-type* | intact | 19 (38) | 5 (10) | 26 (52) | collective | 14 (78) | 4 (22) | 0 (0) | epithelial |
| PCO17 | wild-type | intact | 1 (4) | 11 (44) | 13 (52) | mesenchymal | 2 (18) | 1 (9) | 8 (73) | mesenchymal |
| PCO18 | missense | intact | 25 (51) | 8 (16) | 16 (33) | collective | 25 (83) | 1 (3) | 4 (13) | epithelial |
| PCO19 | wild-type | intact | 19 (24) | 37 (47) | 23 (29) | mesenchymal | 11 (44) | 11 (44) | 3 (12) | mesenchymal |
| PCO20 | wild-type | intact | 1 (2) | 28 (61) | 17 (37) | mesenchymal | 2 (14) | 10 (71) | 2 (14) | mesenchymal |
| PCO21 | wild-type | lost | 15 (31) | 23 (48) | 10 (21) | mesenchymal | 8 (31) | 14 (54) | 4 (15) | mesenchymal |
| PCO22 | deletion | lost | 31 (53) | 25 (42) | 3 (5) | collective | 18 (62) | 10 (34) | 1 (3) | epithelial |
| PCO23 | deletion | lost | 26 (43) | 10 (17) | 24 (40) | collective | 13 (54) | 8 (33) | 3 (13) | epithelial |
| PCO24 | wild-type | intact | 0 (0) | 62 (78) | 18 (23) | mesenchymal | 3 (11) | 11 (39) | 14 (50) | mesenchymal |
| PCO25 | wild-type | intact | 3 (8) | 32 (80) | 5 (13) | mesenchymal | 2 (33) | 4 (67) | 0 (0) | mesenchymal |
*
Although wild-type for SMAD4, a mutation was identified in TGFBR2.