Table 2.
Primary Tumor Features in PDAC Organoid Cohort
| Primary Tumor Morphological Analysis | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Growth Pattern | Expression by IHC | ||||||||
| SMAD4 Mutation Status | Organoid Invasion Pattern | Glandular (%) | Single Cell (%) | ECAD (%) | VIM (%) | P40 | GATA6 | Mesenchymal Score | |
| PCO1 | wild-type | mesenchymal | 80 | 20 | 100 | 0 | negative | high | 0 |
| PCO2 | wild-type | mesenchymal | 70 | 30 | 100 | 0 | focal | low | 2 |
| PCO3 | frameshift | collective | 90 | 10 | 100 | 0 | focal | moderate | 1 |
| PCO4 | deletion | collective | 70 | 30 | 100 | 0 | focal | high | 1 |
| PCO5 | wild-type | mesenchymal | 90 | 10 | 100 | 70 | focal | low | 3 |
| PCO6 | wild-type | mesenchymal | 90 | 10 | 100 | 10 | negative | low | 2 |
| PCO7 | deletion | collective | 80 | 20 | 100 | 0 | negative | high | 0 |
| PCO8 | wild-type | mesenchymal | 90 | 10 | 100 | 0 | negative | high | 0 |
| PCO9 | wild-type | mesenchymal | 100 | 0 | 100 | 0 | negative | high | 0 |
| PCO10 | deletion | collective | 80 | 20 | 100 | 0 | negative | high | 0 |
| PCO11 | wild-type | mesenchymal | 70 | 30 | 100 | 0 | focal | high | 1 |
| PCO12 | deletion | mesenchymal | 90 | 10 | 100 | 0 | focal | high | 1 |
| PCO13 | wild-type | mesenchymal | 90 | 10 | 100 | 0 | focal | high | 1 |
| PCO14 | deletion | mesenchymal | 70 | 30 | 90 | 0 | focal | high | 2 |
| PCO15 | wild-type | mesenchymal | 90 | 10 | 90 | 0 | negative | high | 1 |
| PCO16 | wild-type* | collective | 90 | 10 | 100 | 0 | negative | high | 0 |
| PCO17 | wild-type | mesenchymal | 100 | 0 | 100 | 0 | negative | high | 0 |
| PCO18 | missense | collective | 80 | 20 | 100 | 0 | negative | moderate | 0 |
| PCO19 | wild-type | mesenchymal | 60 | 40 | 90 | 10 | negative | high | 2 |
| PCO20 | wild-type | mesenchymal | 40 | 60 | 100 | 0 | negative | low | 1 |
| PCO21 | wild-type | mesenchymal | 90 | 10 | 80 | 0 | negative | low | 2 |
| PCO22 | deletion | collective | 50 | 50 | 90 | 0 | negative | high | 1 |
| PCO23 | deletion | collective | 90 | 10 | 100 | 0 | negative | moderate | 0 |
| PCO24 | wild-type | mesenchymal | 50 | 50 | 100 | 10 | focal | high | 2 |
| PCO25 | wild-type | mesenchymal | 90 | 10 | 90 | 0 | focal | high | 2 |
| p-value+ | - | 0.58 | 0.62 | 0.27 | 0.40 | 0.14 | 0.020 | ||
*
Although wild-type for SMAD4, a mutation was identified in TGFBR2. +p value comparing PDACs giving rise to predominantly collective vs mesenchymal organoids, calculated using Mann-Whitney U Test (growth pattern, mesenchymal score) or Fisher’s exact test (all others).