Table 3.

Comparison of FAD2 transcription and linoleate composition between growth and lipid accumulation phases. FAD2 transcript levels were measured by qPCR and fold-change between timepoints was calculated using the 2^−ΔΔCt method (Livak and Schmittgen 2001). Ratios of percent linoleate composition were calculated by dividing percent linoleate values at 16 h by those at 96 h using the data presented in Fig. 1a. ND: not detected.

Strain	Ratio of FAD2 transcript at 16 h / 96 h	Ratio of linoleate composition at 16 h / 96 h
p1-FAD2	178	15
p2-FAD2	0.3	ND
p3-FAD2	8.8	14
p4-FAD2	2.6	7
p5-FAD2	1855	18
Δfad2	ND	ND
YB-392 (wt)	1.1	4.5