Table 4.

Comparison of OLE1 transcription and Δ9 desaturated fatty acid composition between growth and lipid accumulation phases. OLE1 transcript levels were measured by qPCR and fold-change between timepoints was calculated using the 2^−ΔΔCt method (Livak and Schmittgen 2001). Ratios of percent desaturated fatty acid composition were calculated by dividing percent fatty acid values at 16 h by those at 96 h using the data presented in Fig. 2a.

Strain	Ratio of OLE1 transcript at 16 h / 96 h	Ratio of Δ9 product composition at 16 h / 96 h
p1-OLE1	63	1.8
p4-OLE1	63	1.8
p5-OLE1	688	1.8
YB-392 (wt)	1.2	1.1