Fig. 1.

ENZ and ODM inhibit proliferation of LNCaP and C4-2 cells. a Western blotting analysis reveals that IL-23 receptor (IL-23R) is expressed in both androgen-dependent LNCaP and castration-resistant C4-2 cells detecting three isoforms. α-Tubulin serves as loading control on the same membrane. b–e Growth assays were performed with LNCaP and C4-2 cells. Cells were treated for 3 days (b, c) or for 6 days (d, e) in the presence of ENZ (10 μM) or ODM (10 μM) in the absence or presence of 100 ng/ml recombinant human IL-23. Proliferation was analyzed by crystal violet staining with subsequent measurement of absorbance at 590 nm. As solvent control, DMSO was used. n = 3; *, p < 0.05; **, p < 0.01; ***, p < 0.001