Fig. 2.

ENZ and ODM inhibit the AR target genes KLK3 and FKBP5 in both LNCaP and C4-2 cells. LNCaP and C4-2 cells were treated with or without IL-23 (100 ng/ml) for an hour prior the treatment of indicated compounds DMSO (0.1%), ENZ (10 μM), and ODM (10 μM) for further 72 h. RNA was extracted and subjected to RT-qPCR. As housekeeping gene, TBP was used for normalization of the mRNA levels of LNCaP cell’s (a) FKBP5, (b) KLK3, and C4-2 cell’s (c) FKBP5 and (d) KLK3. The DMSO control was set arbitrarily as 1 and the obtained values are depicted relative to DMSO control. n = 4; *, p < 0.05; **, p < 0.01; ***, p < 0.001