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. 2020 Jun 20;11(3-4):182–190. doi: 10.1007/s12672-020-00391-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — IL23 reduces ENZ-induced CDKN2A mRNA levels in C4-2 cells. The experiments were performed as described in Fig. 2. mRNA levels of CDKN2A encoding p16 were analyzed from a LNCaP and b C4–2 extracts by RT-qPCR. Protein levels of p16 of c LNCaP and d C4-2 cells were analyzed by Western blotting and normalized to β-Actin levels. The DMSO control was set arbitrarily as 1 and the obtained values are depicted relative to DMSO control