Figure 1. Chk1 inhibitors reduce expression of Chk1 and induce DNA damage.

(A) λ820 mouse lymphoma cells were treated with LY2603618 for 24 h, and cells were analyzed by immunoblotting for Chk1 and loading control β-actin. (B) λ820 mouse lymphoma cells were treated with CCT245737 and cells were analyzed by immunoblotting for apoptosis marker cleaved PARP, total or phosphorylated (p)Chk1 and DNA damage marker phosphorylated histone 2AX (γH2Ax). (C) λ820 mouse lymphoma cells were treated with LY2603618 or ATR inhibitor VE821 for 24 h, and cells were analyzed by immunoblotting for total Chk1, pChk1, phosphorylated (p)RPA2, and loading control β-actin.

Source data are available for this figure.