Figure 1. Ngf Expression Domains and Innervation of the Uninjured Calvarium.

(A) Tile scan of the uninjured parietal bone and sagittal suture (center) in the NGF-eGFP reporter animal.

(B–D) High-magnification images of cranial sutures within NGF-eGFP reporter animals, including (B) patent sagittal suture, (C) patent coronal suture, and (D) fused portion of posterofrontal suture.

(E) NGF reporter activity within the intact frontal bone. PO, periosteum; DM, dura mater.

(F and G) Immunohistochemical staining (appearing red) within the sagittal suture of NGF-eGFP reporter animals, including (F) Gli1 and (G) Axin2.

(H) PGP9.5 whole-mount image of the patent sagittal suture. White dashed lines indicate margins of the patent suture, imaged from the endocranial aspect.

(I and J) TUBB3 (beta III tubulin) whole-mount images of patent cranial sutures, including the (I) sagittal and (J) coronal suture. White dashed lines indicate margins of the patent suture, imaged from the endocranial aspect.

(K and L) Immunohistochemical staining typifying nerve fibers within the sagittal suture, including (K) CGRP⁺ peptidergic fibers (appearing red) (L) and TH⁺ sympathetic fibers (appearing cyan). White dashed lines indicate margins of the patent suture, imaged from the endocranial aspect.

(M) Tile scan of TUBB3⁺ nerve fibers in the uninjured frontal bone. TUBB3⁺ nerve fibers appear green.

(N–P) Cross-sectional images of nerve fibers within patent cranial sutures using pan-neuronal Thy1-YFP transgenic reporter animals. Thy1-YFP reporter activity within patent cranial sutures, including (N) sagittal, (O) coronal, and (P) lambdoid sutures. Thy1-YFP reporter activity appears green.

DAPI counterstain appears blue in all images. Representative images shown from n = 4 mice. All white scale bar, 50 μm. See also Figure S1.