. Author manuscript; available in PMC: 2020 Dec 10.

Published in final edited form as: J Am Chem Soc. 2020 Jun 1;142(23):10506–10515. doi: 10.1021/jacs.0c03535

Table 1.

Steady-state kinetic constants of CndF.

substrate	k_cat (s⁻¹)	K_M (mM)	k_cat/K_M (M⁻¹ s⁻¹)
O-acetyl-l-homoserine 9	0.086 ± 0.014	0.48 ± 0.003	1.8 x 10²
acetoacetate 10	0.064 ± 0.012	2.8 ± 0.9	22
ethyl acetoacetate 8a	0.027 ± 0.002	1.9 ± 0.2	14