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. 2019 Oct 19;28(23):3982–3996. doi: 10.1093/hmg/ddz245

Figure 7.

Figure 7

Abnormalities in the nuclear lamina structure in dopaminergic neurons of PD patients. (A) Substantia nigra from control, idiopathic PD and LRRK2 G2019S PD patients were processed for immunohistochemistry with anti-lamin A/C. Scale bar, 20 μm (first and second panels) and 10 μm (third to fifth panels). Arrows indicate the nuclear lamina of neuromelanin-containing neurons. Misshapen nuclear lamina of neuromelanin-containing neurons is indicated with arrowheads. (B) The graph represents the percent of dopaminergic neurons with abnormal lamina structure characterized by accentuated folds and localized thickness. Values represent the mean ± S.E.M. Substantia nigra of three control and three PD patients were analyzed, and 18–40 dopaminergic neurons were scored for every patient. Unpaired two-tailed Student’s t test (P < 0.001). (C) Frontal cortex from control, idiopathic PD and LRRK2 G2019S PD patients were processed for immunohistochemistry with anti-lamin A/C. Scale bar, 20 μm. Arrows indicate the nuclear lamina of neuromelanin-containing neurons. Misshapen nuclear lamina of neuromelanin-containing neurons is indicated with arrowheads. (D) The graph represents the percent of cortical neurons with abnormal lamina. Values represent the mean ± S.E.M. Cortici of four control, four idiopathic PD and three LRRK2 G2019S PD patients were analyzed, and an average of 90–105 cortical neurons were scored from each patient group. Repeated-measures one-way ANOVA with Bonferroni post hoc test (P < 0.0001).