Table 1. Number of SSRs, polymorphic SSRs frequency of two verified species.
| Species for verification | ||
|---|---|---|
| Rice | Grass carp | |
| Source of the transcriptome | SRR1799209 | SRR1618540 |
| SRR1974265 | SRR1618542 | |
| SRR2048540 | ||
| Total number of SSRs in transcriptome1 (detected by MISA) |
9,901 | 16,700 |
| Number of putatively polymorphic SSRs enriched by this program | 4802 | 6802 |
| Number of primers designed for validation experiments | 30 | 20 |
| Number of primers amplified with clear product bands3 (%) | 27 (90%) | 20 (100%) |
| Number of polymorphic SSRs4 (%) | 265 (96.30%) | 195 (95.00%) |
Notes:
1
The mean number of SSRs in the transcriptome (except mononucleotide) SSRs.
2
The putative polymorphic SSRs enriched by this program were showed in Appendix File 1.
3
The percentage of primers that can be amplified with clear products bands.
4
The percentage of SSRs that are polymorphic.
5
The detail information of polymorphic SSRs validated by experiments was showed in Appendix File 2.