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. 2020 Mar 11;9(6):e010240. doi: 10.1161/JAHA.118.010240

Figure 5.

Figure 5

mPinX1t increased the ratio of cytosolic Gata4 mRNAs to nuclear Gata4 mRNAs; knockdown of Nup133 abolished the effect. A, Gata4 and empty vector/mPinX1/mPinX1t were overexpressed in HEK293FT, followed by fractionation of cytosolic and nuclear mRNAs. RT‐qPCR on the Gata4 mRNAs was then performed using the cytosolic and the nuclear fractions. The amount of Gata4 transcripts in cytosol was normalized to that in nucleus. Our results showed that, in mPinX1t and Gata4 co‐transfected group, a higher ratio of cytosolic Gata4 mRNAs to nuclear Gata4 mRNAs was detected when compared with that in control group (empty vector and Gata4 co‐transfected group). On the other hand, mPinX1 and Gata4 co‐transfected group tended to have a lower ratio of cytosolic Gata4 mRNAs to nuclear Gata4 mRNAs. Data were presented as mean±SEM (n=3; where n represents independent experiments). *P<0.05 vs control group. ## P<0.01 vs mPinX1 group. B, Gata4, empty vector/mPinX1/mPinX1t and siRNA against Nup133 (siNup133) were overexpressed in HEK293FT, followed by fractionation of cytosolic and nuclear mRNAs. RT‐qPCR on the Gata4 mRNAs was then performed using the cytosolic and the nuclear fractions. The amount of Gata4 transcripts in cytosol was normalized to that in nucleus. Our results showed that there was no statistical difference in the ratio of cytosolic Gata4 mRNAs to nuclear Gata4 mRNAs between different groups. Data were presented as mean±SEM (n=3; where n represents independent experiments).